Construction of GPx Active Centers on Natural Protein Nanodisk/Nanotube: A New Way to Develop Artificial Nanoenzyme
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- 作者：Chunxi Hou ; Quan Luo ; Jinliang Liu ; Lu Miao ; Chunqiu Zhang ; Yuzhou Gao ; Xiyu Zhang ; Jiayun Xu ; Zeyuan Dong ; Junqiu Liu
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：October 23, 2012
- 年：2012
- 卷：6
- 期：10
- 页码：8692-8701
- 全文大小：533K
- 年卷期：v.6,no.10(October 23, 2012)
- ISSN：1936-086X
文摘
Construction of catalytic centers on natural protein aggregates is a challenging topic in biomaterial and biomedicine research. Here we report a novel construction of artificial nanoenzyme with glutathione peroxidase (GPx)-like function. By engineering the surface of tobacco mosaic virus (TMV) coat protein, the main catalytic components of GPx were fabricated on TMV protein monomers. Through direct self-assembly of the functionalized viral coat proteins, the multi-GPx centers were installed on these well-defined nanodisks or nanotubes. With the help of muti-selenoenzyme centers, the resulting organized nanoenzyme exhibited remarkable GPx activity, even approaching the level of natural GPx. The antioxidation study on subcell mitochondrial level demonstrated that virus-based nanoenzyme exerted excellent capacity for protecting cell from oxidative damage. This strategy represents a new way to develop artificial nanoenzymes.