Identification of Sinensetin Metabolites in Rat Urine by an Isotope-Labeling Method and Ultrahigh-Performance Liquid Chromatography鈥揈lectrospray Ionization Mass Spectrometry
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- 作者：Guor-Jien Wei ; Jenn-Feng Sheen ; Wen-Chien Lu ; Lucy Sun Hwang ; Chi-Tang Ho ; Ching-I Lin
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：May 29, 2013
- 年：2013
- 卷：61
- 期：21
- 页码：5016-5021
- 全文大小：363K
- 年卷期：v.61,no.21(May 29, 2013)
- ISSN：1520-5118
文摘
Sinensetin (SIN), one of the major polymethoxyflavones (PMFs) contained mainly in the citrus peels, has been reported to possess various bioactivities, including antifungal, antimutagenic, anticancer, and anti-inflammatory activities. Although the biotransformation of SIN in fungi and insects has been reported, the information about the metabolism of SIN in mammals is still unclear. In this study, formation of SIN metabolites in rats was investigated. Four isotope-labeled SINs ([4鈥?D<sub>3sub>]SIN, [3鈥?D<sub>3sub>]SIN, [5-D<sub>3sub>]SIN, and [6-D<sub>3sub>]SIN) were synthesized and administered to rat. The urine samples were collected and main metabolites were monitored by ultrahigh-performance liquid chromatography鈥揺lectrospray ionization mass spectrometry. The administered compound and four SIN metabolites were detected in rat urine. These metabolites were identified as 4鈥?hydroxy-5,6,7,3鈥?tetramethoxyflavone, 5-hydroxy-6,7,3鈥?4鈥?tetramethoxyflavone, 6-hydroxy-5,7,3鈥?4鈥?tetramethoxyflavone, and 7-hydroxy-5,6,3鈥?4鈥?tetramethoxyflavone sulfate.