Biosensor Medaka for Monitoring Intersex Caused by Estrogenic Chemicals

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• 作者：Yanbin Zhao ; Chen Wang ; Shuang Xia ; Jieqiong Jiang ; Rui Hu ; Guanxiang Yuan ; Jianying Hu
• 刊名：Environmental Science & Technology
• 出版年：2014
• 出版时间：February 18, 2014
• 年：2014
• 卷：48
• 期：4
• 页码：2413-2420
• 全文大小：403K
• ISSN：1520-5851

文摘

Estrogenic chemicals can induce intersex in fish species leading to disturbance of spermatogenesis and impairment of reproductive success. To overcome the shortcomings of conventional histopathological observation on intersex (low sensitivity, relatively poor accuracy, long experimental periods, as well as laborious and time-consuming), we generated a pMOSP1-EGFP transgenic medaka fish model. In this transgenic fish, the green fluorescence protein (GFP) reporter gene was derived by the regulatory elements of the OSP1 gene, which is a specific and sensitive molecular biomarker for indicating intersex occurrence in male medaka fish exposed to estrogenic chemicals. The transgenic GFP was faithfully expressed in ovaries and in testes with intersex, perfectly mimicking the expression pattern of endogenous OSP1. In intersex testis, the diameters of primary oocytes which could be distinguished by GFP fluorescence observation were as small as 10 渭m, lower than that (more than 20 渭m) which is observable by histopathology. Using the novel transgenic medaka fish, intersex was observed after 90-day exposure to 0.75 ng/L 17伪-ethinyloestradiol (EE₂) (0鈥?0 dph), but only at concentrations of at least 1.38 ng/L EE₂ by histopathology. An effectiveness of a short-term in vivo assay for screening estrogenic chemicals that can monitor intersex appearance at early sex developmental stage (about 30 dph) in male medaka fish was also demonstrated by assessing the intersex induction of EE₂, 17尾-estradiol and 4-nonylphenol. This newly developed assay provides an enhanced ability for screening and testing estrogenic chemicals with the potential to induce intersex and studying their biological impacts.