文摘
To surmount the challenges of the locus determination and accurate quantification of 5-methyl-2鈥?deoxycytidine (5MedC) in DNA fragments that contain multiple 5MedC residues, we designed and synthesized two N-halogeno-N-sodiobenzenesulfonamide reagents that provide a new chemical method for probing 5MedC in DNA sequences. When the strategy we provided was combined with 尾-glucosyltransferase, 5MedC could be distinguished from 5-hydroxymethyl-2鈥?deoxycytidine (5hmdC) and deoxycytidine (dC) through the introduction of a glucose moiety to the hydroxyl group of 5hmdC.