Constant-Volume Hydrogel Osmometer: A New Device Concept for Miniature Biosensors

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• 作者：In Suk Han ; Man-Hee Han ; Jinwon Kim ; Seok Lew ; Young Jun Lee ; Ferenc Horkay ; and Jules J. Magda
• 刊名：Biomacromolecules
• 出版年：2002
• 出版时间：November 2002
• 年：2002
• 卷：3
• 期：6
• 页码：1271 - 1275
• 全文大小：90K
• 年卷期：v.3,no.6(November 2002)
• ISSN：1526-4602

文摘

A new type of biosensor is proposed that combines the recognition properties of "intelligent" hydrogelswith the sensitivity and reliability of microfabricated pressure transducers. In the proposed device, analyte-induced changes in the osmotic swelling pressure of an environmentally responsive hydrogel are measuredby confining it within a small implantable enclosure between a rigid semipermeable membrane and thediaphragm of a miniature pressure transducer. Proof-of-principle tests of this device were performed invitro using pH-sensitive hydrogels, with osmotic deswelling data for the same hydrogels used as a benchmarkfor comparison. The swelling pressure of the hydrogel was accurately determined from osmotic deswellingmeasurements against reservoirs of known osmotic stress. Values of swelling pressure vs salt concentrationmeasured with a preliminary version of the sensor agree well with osmotic deswelling results. Throughmodification of the hydrogel with various enzymes or pendant binding moieties, the sensor has the potentialto detect a wide range of biological analytes with good specificity.