Novel Xylanase from a Holstein Cattle Rumen Metagenomic Library and Its Application in Xylooligosaccharide and Ferulic Acid Production from Wheat Straw
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- 作者：Fansheng Cheng ; Jiping Sheng ; Rubo Dong ; Yejun Men ; Lin Gan ; Lin Shen
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：December 26, 2012
- 年：2012
- 卷：60
- 期：51
- 页码：12516-12524
- 全文大小：593K
- 年卷期：v.60,no.51(December 26, 2012)
- ISSN：1520-5118
文摘
A novel gene fragment containing a xylanase was identified from a Holstein cattle rumen metagenomic library. The novel xylanase (Xyln-SH1) belonged to the glycoside hydrolase family 10 (GH10) and exhibited a maximum of 44% identity to the glycoside hydrolase from Clostridium thermocellum ATCC 27405. Xyln-SH1 was heterologously expressed, purified, and characterized. A high level of activity was obtained under the optimum conditions of pH 6.5 and 40 掳C. A substrate utilization study indicated that Xyln-SH1 was cellulase-free and strictly specific to xylan from softwood. The synergistic effects of Xyln-SH1 and feruloyl esterase (FAE-SH1) were observed for the release of xylooligosaccharides (XOS) and ferulic acid (FA) from wheat straw. In addition, a high dose of Xyln-SH1 alone was observed to improve the release of FA from wheat straw. These features suggest that this enzyme has substantial potential to improve biomass degradation and industrial applications.