Adsorption and Self-Assembly of M13 Phage into Directionally Organized Structures on C and SiO2 Films

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• 作者：Pouya Moghimian ; Vesna Srot ; Dirk Rothenstein ; Sandra J. Facey ; Ludger Harnau ; Bernhard Hauer ; Joachim Bill ; Peter A. van Aken
• 刊名：Langmuir
• 出版年：2014
• 出版时间：September 30, 2014
• 年：2014
• 卷：30
• 期：38
• 页码：11428-11432
• 全文大小：352K
• ISSN：1520-5827

文摘

A versatile method for the directional assembly of M13 phage using amorphous carbon and SiO₂ thin films was demonstrated. A high affinity of the M13 phage macromolecules for incorporation into aligned structures on an amorphous carbon surface was observed at the concentration range, in which the viral nanofibers tend to disorder. In contrast, the viral particles showed less freedom to adopt an aligned orientation on SiO₂ films when deposited in close vicinity. Here an interpretation of the role of the carbon surface in significant enhancement of adsorption and generation of viral arrays with a high orientational order was proposed in terms of surface chemistry and competitive electrostatic interactions. This study suggests the use of amorphous carbon substrates as a template for directional organization of a closely-packed and two-dimensional M13 viral film, which can be a promising route to mineralize a variety of smooth and homogeneous inorganic nanostructure layers.