MALDI-TOF Mass Spectrometry of Insoluble Giant Polycyclic Aromatic Hydrocarbons by a New Method of Sample Preparation

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• 作者：Laurence Przybilla ; Johann-Diedrich Brand ; Kimihiro Yoshimura ; Hans Joachim R&auml ; der ; and Klaus Mü ; llen
• 刊名：Analytical Chemistry
• 出版年：2000
• 出版时间：October 1, 2000
• 年：2000
• 卷：72
• 期：19
• 页码：4591 - 4597
• 全文大小：117K
• 年卷期：v.72,no.19(October 1, 2000)
• ISSN：1520-6882

文摘

The insolubility of giant polycyclic aromatic hydrocarbons(PAHs) prevents their characterization by conventionalanalytical methods, which require a solubilization of theanalyte. Laser desorption mass spectrometry may be usedto analyze insoluble samples but is limited to relativelylow molecular weights (~2000), in the case of PAHs. Toovercome this limitation, we applied MALDI-TOF massspectrometry. Since MALDI sample preparation alsorequires solubility of analyte and matrix molecules, thesample preparation needed modification. The giant PAHs(>2000 Da) were investigated after using a new samplepreparation, consisting of mechanically mixing analyteand matrix without any solubilization procedures. Thissolvent-free process allows insoluble compounds to becharacterized. Furthermore, new organic molecules canbe used as a matrix. Indeed, 7,7,8,8-tetracyanoquinodimethane, a new matrix with promising properties, hasproven to be particularly suitable for the measurement ofPAHs. Thanks to the successful characterization withMALDI-TOF mass spectrometry, the chemical design ofgiant PAHs, which was hindered until now for a lack ofanalytical methods, can now continue to develop.