Model Studies on Chemical and Textural Modifications in Gelatin Films by Reaction with Glyoxal and Glycolaldehyde

详细信息    查看全文

• 作者：Robert Spanneberg ; Franziska Osswald ; Igor Kolesov ; Werner Anton ; Hans-Joachim Radusch ; Marcus A. Glomb
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：March 24, 2010
• 年：2010
• 卷：58
• 期：6
• 页码：3580-3585
• 全文大小：746K
• 年卷期：v.58,no.6(March 24, 2010)
• ISSN：1520-5118

文摘

The present study investigated chemically modified gelatin biopolymer films. Gelatin solutions were treated with glyoxal and glycolaldehyde, respectively, at concentrations ranging from 0.25 to 7.5 wt % based on gelatin. From these solutions, films were produced under defined conditions and characterized with different chemical and physical methods. N^ε-carboxymethyllysine (CML), glyoxal-derived lysine dimer (GOLD), and 5-(2-imino-5-oxo-1-imidazolidinyl)norvaline (imidazolinone) were analyzed as chemical parameters for protein modification by reversed-phase high-performance liquid chromatography (RP-HPLC) and fluorescence detection after post-column o-phthaldialdehyde derivatization. An increase in the content of these substances with increasing concentrations of carbonyl modifiers correlated with the loss of available free lysine and arginine residues. Swelling, solubility, and mechanical properties (Young’s modulus, stress and strain at break) showed the relationship with the degree of monovalent modification and cross-linking as well. The determination of unreacted glyoxal and glycolaldehyde suggested a different mechanism of cross-linking induced by glyoxal versus glycolaldehyde as reactive intermediates in Maillard chemistry.