Flow Injection Analysis−Isotope Ratio Mass Spectrometry for Bulk Carbon Stable Isotope Analysis of Alcoholic Beverages

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• 作者：Maik A. Jochmann ; Dirk Steinmann ; Manuel Stephan ; Torsten C. Schmidt
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2009
• 出版时间：November 25, 2009
• 年：2009
• 卷：57
• 期：22
• 页码：10489-10496
• 全文大小：815K
• 年卷期：v.57,no.22(November 25, 2009)
• ISSN：1520-5118

文摘

A new method for bulk carbon isotope ratio determination of water-soluble samples is presented that is based on flow injection analysis−isotope ratio mass spectrometry (FIA-IRMS) using an LC IsoLink interface. Advantages of the method are that (i) only very small amounts of sample are required (2−5 μL of the sample for up to 200 possible injections), (ii) it avoids complex sample preparation procedures such as needed for EA-IRMS analysis (only sample dilution and injection,) and (iii) high throughput due to short analysis times is possible (15 min for five replicates). The method was first tested and evaluated as a fast screening method with industrially produced ethanol samples, and additionally the applicability was tested by the measurement of 81 alcoholic beverages, for example, whiskey, brandy, vodka, tequila, and others. The minimal sample concentration required for precise and reproducible measurements was around 50 μL L⁻¹ ethanol/water (1.71 mM carbon). The limit of repeatability was determined to be r = 0.49%. FIA-IRMS represents a fast screening method for beverage authenticity control. Due to this, samples can be prescreened as a decisive criterion for more detailed investigations by HPLC-IRMS or multielement GC-IRMS measurements for a verification of adulteration.