Combination of Immunosensor Detection with Viability Testing and Confirmation Using the Polymerase Chain Reaction and Culture

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• 作者：Brandy Johnson-White ; Baochuan Lin ; Frances S. Ligler
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：January 1, 2007
• 年：2007
• 卷：79
• 期：1
• 页码：140 - 146
• 全文大小：339K
• 年卷期：v.79,no.1(January 1, 2007)
• ISSN：1520-6882

文摘

Rapid and accurate differential determination of viableversus nonviable microbes is critical for formulation ofan appropriate response after pathogen detection. Sensorsfor rapid bacterial identification can be used for applications ranging from environmental monitoring and homeland defense to food process monitoring, but few provideviability information. This study combines the rapidscreening capability of the array biosensor using animmunoassay format with methods for determination ofviability. Additionally, cells captured by the immobilizedantibodies can be cultured following fluorescence imagingto further confirm viability and for cell population expansion for further characterization, e.g., strain identificationor antibiotic susceptibility testing. Finally, we demonstrateanalysis of captured bacteria using the polymerase chainreaction (PCR). PCR results for waveguide-captured cellswere 3 orders of magnitude more sensitive than thefluorescence immunoassay and can also provide additional genetic information on the captured microbes.These approaches can be used to rapidly detect anddistinguish viable versus nonviable and pathogenic versusnonpathogenic captured organisms, provide culture materials for further analysis on a shorter time scale, andassess the efficacy of decontamination or sterilizationprocedures.