A Self-Assembled Multivalent Pseudopolyrotaxane for Binding Galectin-1
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- 作者：Alshakim Nelson ; Jason M. Belitsky ; Sé ; bastien Vidal ; C. Steven Joiner ; Linda G. Baum ; and J. Fraser Stoddart
- 刊名：Journal of the American Chemical Society
- 出版年：2004
- 出版时间：September 29, 2004
- 年：2004
- 卷：126
- 期：38
- 页码：11914 - 11922
- 全文大小：751K
- 年卷期：v.126,no.38(September 29, 2004)
- ISSN：1520-5126
文摘
A self-assembled pseudopolyrotaxane consisting of lactoside-displaying cyclodextrin (CD) "beads"threaded onto a linear polyviologen "string" was investigated for its ability to inhibit galectin-1-mediatedT-cell agglutination. The CDs of the pseudopolyrotaxane are able to spin around the axis of the polymerchain as well as to move back and forth along its backbone to alter the presentation of its ligand. Thissupramolecular superstructure incorporates all the advantages of polymeric structures, such as the abilityto span large distances, along with a distinctively dynamic presentation of its lactoside ligands to afford aneoglycoconjugate that can adjust to the relative stereochemistries of the lectin's binding sites. Thepseudopolyrotaxane exhibited a valency-corrected 10-fold enhancement over native lactose in theagglutination assay, which was greater than the enhancements observed for lactoside-bearing trivalentglycoclusters and a lactoside-bearing chitosan polymer tested using the same assay. The experimentalresults indicate that supramolecular architectures, such as the pseudopolyrotaxane, provide tools forinvestigating protein-carbohydrate interactions.