Trifluoroethanethiol: An Additive for Efficient One-Pot Peptide Ligation鈭扗esulfurization Chemistry

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• 作者：Robert E. Thompson ; Xuyu Liu ; Noelia Alonso-Garc铆a ; Pedro Jos茅 Barbosa Pereira ; Katrina A. Jolliffe ; Richard J. Payne
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：June 11, 2014
• 年：2014
• 卷：136
• 期：23
• 页码：8161-8164
• 全文大小：295K
• 年卷期：v.136,no.23(June 11, 2014)
• ISSN：1520-5126

文摘

Native chemical ligation followed by desulfurization is a powerful strategy for the assembly of proteins. Here we describe the development of a high-yielding, one-pot ligation鈭抎esulfurization protocol that uses trifluoroethanethiol (TFET) as a novel thiol additive. The synthetic utility of this TFET-enabled methodology is demonstrated by the efficient multi-step one-pot syntheses of two tick-derived proteins, chimadanin and madanin-1, without the need for any intermediary purification.