Antistaphylococcal Nanocomposite Films Based on Enzyme−Nanotube Conjugates

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• 作者：Ravindra C. Pangule ; Sarah J. Brooks ; Cerasela Zoica Dinu ; Shyam Sundhar Bale ; Sharon L. Salmon ; Guangyu Zhu ; Dennis W. Metzger ; Ravi S. Kane ; Jonathan S. Dordick
• 刊名：ACS Nano
• 出版年：2010
• 出版时间：July 27, 2010
• 年：2010
• 卷：4
• 期：7
• 页码：3993-4000
• 全文大小：334K
• 年卷期：0
• ISSN：1936-086X

文摘

Infection with antibiotic-resistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) is one of the primary causes of hospitalizations and deaths. To address this issue, we have designed antimicrobial coatings incorporating carbon nanotube−enzyme conjugates that are highly effective against antibiotic-resistant pathogens. Specifically, we incorporated conjugates of carbon nanotubes with lysostaphin, a cell wall degrading enzyme, into films to impart bactericidal properties against Staphylococcus aureus and Staphylococcus epidermidis. We fabricated and characterized nanocomposites containing different conjugate formulations and enzyme loadings. These enzyme-based composites were highly efficient in killing MRSA (>99% within 2 h) without release of the enzyme into solution. Additionally, these films were reusable and stable under dry storage conditions for a month. Such enzyme-based film formulations may be used to prevent growth of pathogenic and antibiotic-resistant microorganisms on various common surfaces in hospital settings. Polymer and paint films containing such antimicrobial conjugates, in particular, could be advantageous to prevent risk of staphylococcal-specific infection and biofouling.