Detection of Radionuclides in Capillary Electrophoresis Using a Phosphor-Imaging Detector
详细信息 查看全文
- 作者：Scott E. Tracht ; LouAnn Cruz ; Carolyn M. Stobba-Wiley ; and Jonathan V. Sweedler
- 刊名：Analytical Chemistry
- 出版年：1996
- 出版时间：November 15, 1996
- 年：1996
- 卷：68
- 期：22
- 页码：3922 - 3927
- 全文大小：197K
- 年卷期：v.68,no.22(November 15, 1996)
- ISSN：1520-6882
文摘
A capillary electrophoresis (CE) postcolumn radionuclidedetector has been developed that uses a commercialphosphor-imaging detector and has been optimized forlow-energy 
emitters. Eluant from the separationcapillary is deposited on a membrane. Emission from radioactive analytes on the membrane is integrated using thephosphor-imaging system for 10-72 h. Results fromthephosphor-imaging system are converted to conventionalelectropherograms. Modifications to a priorpostcolumnCE deposition system have been accomplished by addinga buffer makeup capillary; this increases the electrolytepH range and improves reproducibility. The limit ofdetection (LOD) for 35S-labeled analytes is 0.13 amol(8.7pM or 0.007 Bq), while the LOD for 32P-labeledanalytesis 4.9 zmol (0.33 pM or 0.002 Bq), with a linear rangefor 35S-Met from 1.5 amol to 1.5 fmol.
emitters. Eluant from the separationcapillary is deposited on a membrane. Emission from radioactive analytes on the membrane is integrated using thephosphor-imaging system for 10-72 h. Results fromthephosphor-imaging system are converted to conventionalelectropherograms. Modifications to a priorpostcolumnCE deposition system have been accomplished by addinga buffer makeup capillary; this increases the electrolytepH range and improves reproducibility. The limit ofdetection (LOD) for 35S-labeled analytes is 0.13 amol(8.7pM or 0.007 Bq), while the LOD for 32P-labeledanalytesis 4.9 zmol (0.33 pM or 0.002 Bq), with a linear rangefor 35S-Met from 1.5 amol to 1.5 fmol.