Synthesis of Conjugates for a Barbiturate Screening Assay

详细信息    查看全文

• 作者：Maciej Adamczyk ; Jonathan Grote ; Jeanine Douglas ; Robert Dubler ; and Charles Harrington
• 刊名：Bioconjugate Chemistry
• 出版年：1997
• 出版时间：May 1997
• 年：1997
• 卷：8
• 期：3
• 页码：281 - 288
• 全文大小：451K
• 年卷期：v.8,no.3(May 1997)
• ISSN：1520-4812

文摘

Novel derivatives of barbiturates functionalized with free carboxylicacids were designed andsynthesized. Coupling of 5-cyclopentyl-5-carboxycrotylbarbituricacid via its active ester to anaminofluorescein derivative produced a fluorescent tracer.Conjugation of the 5-cyclopentenyl-5-carboxyethylbarbituric acid via its mixed anhydride to thyroglobulinallowed for subsequent development of a polyclonal antibody which was evaluated for binding in afluorescence polarizationimmunoassay format with various barbiturates. The binding studiesshowed good cross-reactivity ofa variety of barbiturates containing both aromatic and aliphatic5-substituents with the tested antisera.The relationship between the immunogen architecture, the chemicalstructure of the binding analytes,and the characteristics of the antisera is also presented.