The
develo
pment of
procedures for retros
pective
detection
and qu
antit
ation of ex
posure to
phosgene, b
ased on
adducts to hemoglobin
and
albumin, is
described. U
pon incub
ation of hum
anblood with [
14C]
phosgene (0-750
![](/im<font color=)
ages/entities/mgr.gif">M),
a signific
ant
part of r
adio
activity (0-13%) bec
ame
associ
ated with globin
and
albumin. U
pon Pron
ase digestion of globin, one of the
adducts w
asi
dentified
as the
pent
ape
pti
de O=C-(V-L)-S-P-A, re
presenting
amino
acid residues 1-5 of
![](/im<font color=)
ages/gifch
ars/
al
ph
a.gif" BORDER=0>-globin, with
a hyd
antoin function between N-termin
al v
aline
and leucine. Micro-LC/t
an
demMS
an
alyses of try
ptic
as well
as V8
prote
ase digests i
dentified one of the
adducts to
albumin
as
a ure
a resulting from intr
amolecul
ar bridging of lysine residues 195
and 199. The
adductedtry
ptic fr
agment could be sensitively
an
alyzed by me
ans of micro-LC/t
an
dem MS with multi
ple-re
action monitoring (MRM), en
abling the
detection in hum
an blood of
an in vitro ex
posurelevel of
![](/im<font color=)
ages/entities/ge.gif">1
![](/im<font color=)
ages/entities/mgr.gif">M
phosgene.