Cooperative Action of -Glucanotransferase and Maltogenic Amylase for an Improved Process of Isomaltooligosaccharide (IMO) Production
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- 作者：Hyun-Soo Lee ; Joong-Hyuck Auh ; Hyun-Geun Yoon ; Myo-Jeong Kim ; Jin-Hee Park ; Seung-Suh Hong ; Min-Hyung Kang ; Tae-Jip Kim ; Tae-Wha Moon ; Jung-Wan Kim ; and Kwan-Hwa Park
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2002
- 出版时间：May 8, 2002
- 年：2002
- 卷：50
- 期：10
- 页码：2812 - 2817
- 全文大小：84K
- 年卷期：v.50,no.10(May 8, 2002)
- ISSN：1520-5118
文摘
Maltogenic amylase and 
-glucanotransferase (-GTase) were employed in an effort to develop anefficient process for the production of isomaltooligosaccharides (IMOs). Bacillus stearothermophilusmaltogenic amylase (BSMA) and -GTase from Thermotoga maritima were overexpressed inEscherichia coli using overexpression vectors. An IMO mixture containing 58% of various IMOs wasproduced from liquefied corn syrup by the hydrolyzing and transglycosylation activities of BSMA alone.When BSMA and -GTase were reacted simultaneously, the IMO content increased to 68% andcontained relatively larger IMOs compared with the products obtained by the reaction without -GTase.Time course analysis of the IMO production suggested that BSMA hydrolyzed maltopentaose andmaltohexaose most favorably into maltose and maltotriose and transferred the resulting moleculessimultaneously to acceptor molecules to form IMOs. -GTase transferred donor sugar molecules tothe hydrolysis products such as maltose and maltotriose to form maltopentaose, which was thenrehydrolyzed by BSMA as a favorable substrate.Keywords: Bacillus stearothermophilus maltogenic amylase; isomaltooligosaccharides (IMOs); Thermotoga maritima; -glucanotransferase (-GTase)
-glucanotransferase (-GTase) were employed in an effort to develop anefficient process for the production of isomaltooligosaccharides (IMOs). Bacillus stearothermophilusmaltogenic amylase (BSMA) and -GTase from Thermotoga maritima were overexpressed inEscherichia coli using overexpression vectors. An IMO mixture containing 58% of various IMOs wasproduced from liquefied corn syrup by the hydrolyzing and transglycosylation activities of BSMA alone.When BSMA and -GTase were reacted simultaneously, the IMO content increased to 68% andcontained relatively larger IMOs compared with the products obtained by the reaction without -GTase.Time course analysis of the IMO production suggested that BSMA hydrolyzed maltopentaose andmaltohexaose most favorably into maltose and maltotriose and transferred the resulting moleculessimultaneously to acceptor molecules to form IMOs. -GTase transferred donor sugar molecules tothe hydrolysis products such as maltose and maltotriose to form maltopentaose, which was thenrehydrolyzed by BSMA as a favorable substrate.Keywords: Bacillus stearothermophilus maltogenic amylase; isomaltooligosaccharides (IMOs); Thermotoga maritima; -glucanotransferase (-GTase)