Reversible Positioning of Single Molecules inside Zero-Mode Waveguides
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- 作者：Joseph Larkin ; Mathieu Foquet ; Stephen W. Turner ; Jonas Korlach ; Meni Wanunu
- 刊名：Nano Letters
- 出版年：2014
- 出版时间：October 8, 2014
- 年：2014
- 卷：14
- 期：10
- 页码：6023-6029
- 全文大小：366K
- ISSN：1530-6992
文摘
We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to the base of 70 nm diameter waveguides for interrogation. Rapid and reversible molecular loading is achieved by controlling the voltage across the device. Using this device we demonstrate protein and DNA loading with efficiency that is orders of magnitude higher than diffusion-based molecular loading.