Handles for Fmoc Solid-Phase Synthesis of Protected Peptides
详细信息 查看全文
- 作者：Miriam G贸ngora-Ben铆tez ; Judit Tulla-Puche ; Fernando Albericio
- 刊名：ACS Combinatorial Science
- 出版年：2013
- 出版时间：May 13, 2013
- 年：2013
- 卷：15
- 期：5
- 页码：217-228
- 全文大小：748K
- 年卷期：v.15,no.5(May 13, 2013)
- ISSN：2156-8944
文摘
Protected peptide fragments are valuable building blocks for the assembly of large peptide sequences through fragment condensation approaches, whereas protected peptides are typically synthesized for the preparation of amide-bridge cyclic peptides in solution. Efficient synthesis of both protected peptides and protected peptide fragments by solid-phase peptide synthesis methodology requires handles that attach the growing peptides to the polymeric support and can be cleaved under appropriate conditions, while maintaining intact the side-chain protecting groups. Here, we provide an overview of attachment methods described in the literature for the preparation of protected peptides using Fmoc/tBu chemistry, including the most commonly used acid-labile linkers along with the most recent and sophisticated.