Distinction of Individual Lanthanide Ions with a DNAzyme Beacon Array

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• 作者：Po-Jung Jimmy Huang ; Mahsa Vazin ; Jennifer J. Lin ; Rachel Pautler ; Juewen Liu
• 刊名：ACS Sensor
• 出版年：2016
• 出版时间：June 24, 2016
• 年：2016
• 卷：1
• 期：6
• 页码：732-738
• 全文大小：687K
• ISSN：2379-3694

文摘

Developing chemical probes to distinguish each lanthanide ion is a long-standing challenge. Aside from its analytical applications, solving this problem will also enhance our knowledge in metal ligand design. Using in vitro selection, we previously reported four RNA-cleaving DNAzymes, each with a different activity trend cross the lanthanide series. We herein performed another eight in vitro selection experiments using each and every lanthanide from La³⁺ to Tb³⁺ but excluding the radioactive Pm³⁺. A new DNAzyme named Gd2b was identified and characterized. By labeling this DNAzyme with a fluorophore/quencher pair to create a catalytic beacon, a detection limit of 14 nM Gd³⁺ was achieved. With the same beacon design, all the five lanthanide-specific DNAzymes were used together to form a sensor array. Each lanthanide ion produces a unique response pattern with these five sensors, allowing a pattern-recognition-based linear discriminant analysis (LDA) algorithm to be applied, where separation was achieved between lanthanides and nonlanthanides, light and heavy lanthanides, and for the most part, each lanthanide. These lanthanide-specific DNA molecules are useful for understanding lanthanide coordination chemistry, designing hybrid materials, and developing related analytical probes.