Phenanthroquinolizidine Alkaloids from the Roots of Boehmeria pannosa Potently Inhibit Hypoxia-Inducible Factor-1 in AGS Human Gastric Cancer Cells
详细信息    查看全文
文摘
A bioassay-guided phytochemical investigation on the methanol extract of Boehmeria pannosa, using a HIF-1-mediatedreporter gene assay, led to the isolation of two phenanthroquinolizidine alkaloids, (-)-cryptopleurine (1) and (-)-(15R)-hydroxycryptopleurine (2). The structure of the new compound 2 was determined by spectroscopic methods.Compounds 1 and 2 potently inhibited the hypoxia-induced expression of a reporter gene under the control of a hypoxiaresponse element (HRE) with IC50 values of 8.7 and 48.1 nM, respectively. Furthermore, 1 and 2 suppressed theaccumulation of HIF-1 protein in a dose-dependent manner, but not the HIF-1 protein and inhibited expression ofvascular endothelial growth factor (VEGF) by hypoxia.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700