Fungal Immunomodulatory Protein from Flammulina velutipes Induces Interferon- Production through p38 Mitogen-Activated Protei
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- 作者：Po-Hui Wang ; Chyong-Ing Hsu ; Sheau-Chung Tang ; Yu-Lu Huang ; Jung-Yaw Lin ; and Jiunn-Liang Ko
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：May 5, 2004
- 年：2004
- 卷：52
- 期：9
- 页码：2721 - 2725
- 全文大小：82K
- 年卷期：v.52,no.9(May 5, 2004)
- ISSN：1520-5118
文摘
FIP-fve is a fungal immunomodulatory protein purified from Flammulina velutipes, an edible goldenneedle mushroom thought to possess potent immunomodulatory properties. When examined for itseffects on lymphocytes, FIP-fve exhibited potent mitogenic effects on human peripheral bloodlymphocytes, inducing G1/G0 to S phase proliferation. T cells activated by FIP-fve show significantproduction and secretion of interferon-
(IFN-) associated with intercellular adhesion molecule 1expression but low detectable levels of interleukin-4 in vitro or in vivo. However, SB203580, the p38mitogen-activated protein kinase (p38 MAPK) inhibitor, can fully abolish the production of IFN- inducedby FIP-fve. At the same time, SB203580 only partially prevents the lymphocytes from progressingfrom G1 to S phase of the cell cycle. These findings demonstrate that FIP-fve is a potent T-cellactivator, mediating its effects via cytokine regulation of p38 MAPK. The immunoprophylatic effectsof FIP-fve in Th2-mediated allergic anaphylaxis are believed to be associated with the ability of FIP-fve to enhance activation of IFN--releasing Th1 cells.Keywords: Fungal immunomodulatory protein; IFN-; p38
(IFN-) associated with intercellular adhesion molecule 1expression but low detectable levels of interleukin-4 in vitro or in vivo. However, SB203580, the p38mitogen-activated protein kinase (p38 MAPK) inhibitor, can fully abolish the production of IFN- inducedby FIP-fve. At the same time, SB203580 only partially prevents the lymphocytes from progressingfrom G1 to S phase of the cell cycle. These findings demonstrate that FIP-fve is a potent T-cellactivator, mediating its effects via cytokine regulation of p38 MAPK. The immunoprophylatic effectsof FIP-fve in Th2-mediated allergic anaphylaxis are believed to be associated with the ability of FIP-fve to enhance activation of IFN--releasing Th1 cells.Keywords: Fungal immunomodulatory protein; IFN-; p38