Single-Molecule Kinetics and Super-Resolution Microscopy by Fluorescence Imaging of Transient Binding on DNA Origami
详细信息 查看全文
- 作者：Ralf Jungmann ; Christian Steinhauer ; Max Scheible ; Anton Kuzyk ; Philip Tinnefeld ; Friedrich C. Simmel
- 刊名：Nano Letters
- 出版年：2010
- 出版时间：November 10, 2010
- 年：2010
- 卷：10
- 期：11
- 页码：4756-4761
- 全文大小：417K
- 年卷期：v.10,no.11(November 10, 2010)
- ISSN：1530-6992
文摘
DNA origami is a powerful method for the programmable assembly of nanoscale molecular structures. For applications of these structures as functional biomaterials, the study of reaction kinetics and dynamic processes in real time and with high spatial resolution becomes increasingly important. We present a single-molecule assay for the study of binding and unbinding kinetics on DNA origami. We find that the kinetics of hybridization to single-stranded extensions on DNA origami is similar to isolated substrate-immobilized DNA with a slight position dependence on the origami. On the basis of the knowledge of the kinetics, we exploit reversible specific binding of labeled oligonucleotides to DNA nanostructures for PAINT (points accumulation for imaging in nanoscale topography) imaging with <30 nm resolution. The method is demonstrated for flat monomeric DNA structures as well as multimeric, ribbon-like DNA structures.