Preparation and Evaluation of a Molecularly Imprinted Polymer Derivatized Silica Monolithic Column for Capillary Electrochromatography and Capillary Liquid Chromatography
详细信息 查看全文
- 作者：Junjie Ou ; Xin Li ; Shun Feng ; Jing Dong ; Xiaoli Dong ; Liang Kong ; Mingliang Ye ; Hanfa Zou
- 刊名：Analytical Chemistry
- 出版年：2007
- 出版时间：January 15, 2007
- 年：2007
- 卷：79
- 期：2
- 页码：639 - 646
- 全文大小：258K
- 年卷期：v.79,no.2(January 15, 2007)
- ISSN：1520-6882
文摘
A method for preparation of molecularly imprinted polymer (MIP) derivatized onto the surface of a monolithicsilica capillary column was successfully developed. Thevinyl groups were first introduced onto the silica monolithby immobilization of 
-methacryloxypropyltrimethoxysilane. Then the MIP coating was copolymerized andanchored onto the surface of the silica monolith. Acetonitrile was selected as porogen (solvent). The otherpreparation conditions, such as monomer concentration,temperature, and time of polymerization, were systematically studied. The obtained MIP-derivatized silica monolith using L-tetrahydropalmatine (L-THP) and (5S,11S)-(-)-Tröger's base (S-TB) as the imprinted template,respectively, was characterized in terms of the retentionbehavior of thiourea and toluene. Under the optimizedCEC conditions, baseline enantioseparations of THP andTB were achieved in 4 min though the effective length ofthe columns was 8.5 cm. The result indicates that enoughrecognition sites were on the surface of silica monolith,resulting in strong recognition ability. Compared with aMIP organic monolith, the MIP-derivatized silica monolithexhibits better column efficiency and stability in CEC.Additionally, the comparison of these two kinds of monolithic columns was performed by capillary liquid chromatography. The separation on MIP-derivatized silica monolith was superior to that on the organic monolith.
-methacryloxypropyltrimethoxysilane. Then the MIP coating was copolymerized andanchored onto the surface of the silica monolith. Acetonitrile was selected as porogen (solvent). The otherpreparation conditions, such as monomer concentration,temperature, and time of polymerization, were systematically studied. The obtained MIP-derivatized silica monolith using L-tetrahydropalmatine (L-THP) and (5S,11S)-(-)-Tröger's base (S-TB) as the imprinted template,respectively, was characterized in terms of the retentionbehavior of thiourea and toluene. Under the optimizedCEC conditions, baseline enantioseparations of THP andTB were achieved in 4 min though the effective length ofthe columns was 8.5 cm. The result indicates that enoughrecognition sites were on the surface of silica monolith,resulting in strong recognition ability. Compared with aMIP organic monolith, the MIP-derivatized silica monolithexhibits better column efficiency and stability in CEC.Additionally, the comparison of these two kinds of monolithic columns was performed by capillary liquid chromatography. The separation on MIP-derivatized silica monolith was superior to that on the organic monolith.