Specific Detection of Integrin 伪v尾3 by Light-Up Bioprobe with Aggregation-Induced Emission Characteristics

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• 作者：Haibin Shi ; Jianzhao Liu ; Junlong Geng ; Ben Zhong Tang ; Bin Liu
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：June 13, 2012
• 年：2012
• 卷：134
• 期：23
• 页码：9569-9572
• 全文大小：285K
• 年卷期：v.134,no.23(June 13, 2012)
• ISSN：1520-5126

文摘

Specific bioprobes with fluorescence turn-on response are highly desirable for high contrast biosensing and imaging. In this work, we developed a new generation bioprobe by integrating tetraphenylsilole, a fluorogenic unit with aggregation-induced emission (AIE) characteristic, with cyclic arginine鈥揼lycine鈥揳spartic acid tripeptide (cRGD), a targeting ligand to integrin 伪_v尾₃ receptor. Emission of the AIE probe is switched on upon its specific binding to integrin 伪_v尾₃, which allows quantitative detection of integrin 伪_v尾₃ in solution and real-time imaging of the binding process between cRGD and integrin 伪_v尾₃ on cell membrane. The probe can be used for tracking integrin 伪_v尾₃ and for identifying integrin 伪_v尾₃-positive cancer cells.