Comprehensive Proteomic Analysis of Shigella flexneri 2a Membrane Proteins
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- 作者：Candong Wei ; Jian Yang ; Junping Zhu ; Xiaobing Zhang ; Wenchuan Leng ; Jing Wang ; Ying Xue ; Lilian Sun ; Weijun Li ; Jin Wang ; Qi Jin
- 刊名：Journal of Proteome Research
- 出版年：2006
- 出版时间：August 2006
- 年：2006
- 卷：5
- 期：8
- 页码：1860 - 1865
- 全文大小：123K
- 年卷期：v.5,no.8(August 2006)
- ISSN：1535-3907
文摘
Shigella flexneri is the causative agent of most shigellosis cases in developing countries. We useddifferent proteolytic enzymes to selectively shave the protruding proteins on the surface of purifiedbacterial membrane sheets or vesicles, and recovered peptides were subsequently identified using2-D LC-MS/MS. As a result, a total of 666 proteins were unambiguously assigned, including 159 integralmembrane proteins, 35 outer membrane proteins and 114 proteins previously annotated as hypothetical.The former had an average grand average hydrophobicity score of 0.362 and were predicted to separatewithin a pH range of 4.1-10.6 with molecular mass 8-148 kDa, which represents the largest validatedset of integral membrane proteins in this organism to date. A functional classification revealed that alarge proportion of the identified proteins were involved in cell envelope biogenesis and energyproduction and conversion. For the first time, this work provides a global view of the S. flexneri 2amembrane subproteome.