Multiscale Fabrication of Multiple Proteins and Topographical Structures by Combining Capillary Force Lithography and Microscope Projection Photolithography

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• 作者：Keon Woo Kwon ; Jong-Cheol Choi ; Kahp-Yang Suh ; Junsang Doh
• 刊名：Langmuir
• 出版年：2011
• 出版时间：April 5, 2011
• 年：2011
• 卷：27
• 期：7
• 页码：3238-3243
• 全文大小：874K
• 年卷期：v.27,no.7(April 5, 2011)
• ISSN：1520-5827

文摘

We present new methods that enable the fabrication of multiscale, multicomponent protein-patterned surfaces and multiscale topologically structured surfaces by exploiting the merits of two well-established techniques: capillary force lithography (CFL) and microscope projection photolithography (MPP) based on a protein-friendly photoresist. We further demonstrate that, when hierarchically organized micro- and nanostructures were used as a cell culture platform, human colon cancer cells (cell line SW480) preferentially adhere and migrate onto the area with nanoscale topography over the one with microscale topography. These methods will provide many exciting opportunities for the study of cellular responses to multiscale physicochemical cues.