Urinary Excretion of 3,N4-Etheno-2'-deoxycytidine in Humans as a Biomarker of Oxidative Stress: Association with Cigarette Smoking
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- 作者：Hauh-Jyun Candy Chen ; Chan-Fu Wu ; Chia-Liang Hong ; and Chia-Ming Chang
- 刊名：Chemical Research in Toxicology
- 出版年：2004
- 出版时间：July 2004
- 年：2004
- 卷：17
- 期：7
- 页码：896 - 903
- 全文大小：126K
- 年卷期：v.17,no.7(July 2004)
- ISSN：1520-5010
文摘
Smokers are known to have elevated levels of lipid peroxidation, a form of oxidative stress.Etheno DNA adduct formation can originate from endogenous lipid peroxidation or fromexogenous exposure of carcinogens. Using a modified stable isotope dilution GC/negative ionchemical ionization/MS assay originally developed for urinary 3,N4-ethenocytosine (
Cyt), thenucleoside 3,N4-etheno-2'-deoxycytidine (dCyd) was detected for the first time in human urine.The presence of dCyd in human urine was confirmed by LC/electrospray ionization/tandemMS. Concentrations of dCyd in the 24 h urine samples from healthy individuals notoccupationally exposed to industrial chemicals were in the range between 0 and 0.80 nM. Astatistically significant correlation was established between cigarette smoking and urinaryexcretion of dCyd after being adjusted for creatinine (p = 0.004). Furthermore, the urinarytotal antioxidant capacity was found to correlate inversely with the dCyd levels (r = -0.50,p = 0.02). The results indicate that urinary dCyd may provide a valuable noninvasivebiomarker for oxidative DNA damage.
Cyt), thenucleoside 3,N4-etheno-2'-deoxycytidine (dCyd) was detected for the first time in human urine.The presence of dCyd in human urine was confirmed by LC/electrospray ionization/tandemMS. Concentrations of dCyd in the 24 h urine samples from healthy individuals notoccupationally exposed to industrial chemicals were in the range between 0 and 0.80 nM. Astatistically significant correlation was established between cigarette smoking and urinaryexcretion of dCyd after being adjusted for creatinine (p = 0.004). Furthermore, the urinarytotal antioxidant capacity was found to correlate inversely with the dCyd levels (r = -0.50,p = 0.02). The results indicate that urinary dCyd may provide a valuable noninvasivebiomarker for oxidative DNA damage.