Design of Polydiacetylene-Phospholipid Supramolecules for Enhanced Stability and Sensitivity

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• 作者：Do Hyun Kang ; Ho-Sup Jung ; Jiseok Lee ; Sungbaek Seo ; Jinsang Kim ; Keesung Kim ; Kahp-Yang Suh
• 刊名：Langmuir
• 出版年：2012
• 出版时间：May 15, 2012
• 年：2012
• 卷：28
• 期：19
• 页码：7551-7556
• 全文大小：384K
• 年卷期：v.28,no.19(May 15, 2012)
• ISSN：1520-5827

文摘

We present polydiacetylene (PDA) liposome assemblies with various phospholipids that have different headgroup charges and phase transition temperatures (T_m). 10,12-Pentacosadiynoic acid (PCDA)-epoxy was used as a base PDA monomer and the insertion of highly charged phospholipids resulted in notable changes in the size of liposome and reduction of the aggregation of PDA liposome. Among the various phospholipids, the phospholipid with a moderate T_m demonstrated enhanced stability and sensitivity, as measured by the size and zeta potential over storage time, thermochoromic response, and transmission electron microscopy images. By combining these results, we were able to detect immunologically an antibody of bovine viral diarrhea virus over a wide dynamic range of 0.001 to 100 渭g/mL.