Self-Assembling Peptide Inspired by a Barnacle Underwater Adhesive Protein

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• 作者：Masahiro Nakano ; Jian-Ren Shen ; Kei Kamino
• 刊名：Biomacromolecules
• 出版年：2007
• 出版时间：June 2007
• 年：2007
• 卷：8
• 期：6
• 页码：1830 - 1835
• 全文大小：188K
• 年卷期：v.8,no.6(June 2007)
• ISSN：1526-4602

文摘

An underwater bioadhesive generally comprises a multiprotein complex that provides a molecular basis for self-assembly. We report here a new class of self-assembling peptide inspired by a 20 kDa barnacle cement protein.Studies on the chemically synthesized 24-residue peptide have revealed that (1) it underwent irreversible self-assembly upon the addition of salt, (2) the self-assembly was started at a salt concentration close to that of seawaterwith noncovalent intermolecular interactions, (3) the self-assembled material resembled a macroscopic membraneof interwoven nanofilaments, (4) incubation in an alkaline pH range formed the intramolecular disulfide bond ofa peptide molecule, thus triggering a conformation change of the molecule, and (5) conformational change of thebuilding block promoted the formation of a nanofiber, resulting in the display of a three-dimensional meshlikemesoscopic structure with defined pores having a diameter of approximately 200 nm. The peptide is likely toprovide a suitable basis for further development of peptide-based materials.