Neutron Crystallographic Studies Reveal Hydrogen Bond and Water-Mediated Interactions between a Carbohydrate-Binding Module and Its Bound Carbohydrate Ligand

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• 作者：S. Zo毛 Fisher ; Laura von Schantz ; Maria H氓kansson ; Derek T. Logan ; Mats Ohlin
• 刊名：Biochemistry
• 出版年：2015
• 出版时间：October 27, 2015
• 年：2015
• 卷：54
• 期：42
• 页码：6435-6438
• 全文大小：248K
• ISSN：1520-4995

文摘

Carbohydrate-binding modules (CBMs) are key components of many carbohydrate-modifying enzymes. CBMs affect the activity of these enzymes by modulating bonding and catalysis. To further characterize and study CBM鈥搇igand binding interactions, neutron crystallographic studies of an engineered family 4-type CBM in complex with a branched xyloglucan ligand were conducted. The first neutron crystal structure of a CBM鈥搇igand complex reported here shows numerous atomic details of hydrogen bonding and water-mediated interactions and reveals the charged state of key binding cleft amino acid side chains.