NTP Concentration Switches Transcriptional Activity by Changing the Large-Scale Structure of DNA

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• 作者：Tatsuo Akitaya ; Kanta Tsumoto ; Ayako Yamada ; Naoko Makita ; Koji Kubo ; and Kenichi Yoshikawa
• 刊名：Biomacromolecules
• 出版年：2003
• 出版时间：September 2003
• 年：2003
• 卷：4
• 期：5
• 页码：1121 - 1125
• 全文大小：94K
• 年卷期：v.4,no.5(September 2003)
• ISSN：1526-4602

文摘

It is becoming clearer that genetic activity is closely associated with the intracellular energy state. However,the mechanisms of this association are still unclear. In this study, we focused on large-scale changes in thestructure of DNA to examine the effect of the NTP concentration on the transcription reaction with T7RNA polymerase and compared the results with long duplex DNA to those with a short persistent-length¹fragment. The transcriptional activity dramatically changed only for long duplex DNA within a narrowrange of NTP concentrations associated with changes in the large-scale structure of DNA. This result suggeststhat the energy state may play an essential role in regulating ON/OFF switching on transcriptional activity.