Potentiometric Detection of DNA Hybridization

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• 作者：Apon Numnuam ; Karin Y. Chumbimuni-Torres ; Yun Xiang ; Ralph Bash ; Panote Thavarungkul ; Proespichaya Kanatharana ; Ern&ouml ; Pretsch ; Joseph Wang ; Eric Bakker
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：January 16, 2008
• 年：2008
• 卷：130
• 期：2
• 页码：410 - 411
• 全文大小：60K
• 年卷期：v.130,no.2(January 16, 2008)
• ISSN：1520-5126

文摘

The use of potentiometric microsensors is demonstrated here for the first time to detect DNA hybridization. Cadmium sulfide nanocrystal labels bound on a secondary oligonucleotide are dissolved and detected with cadmium-selective microelectrodes exhibiting DNA detection limits of ca. 2 fmol in a 200 L sample.