Exploring Fluorescence Antibunching in Solution To Determine the Stoichiometry of Molecular Complexes
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- 作者：Jan S
//pubs.acs.org/images/entities/yacute.gif" border="0">kora ; Karin Kaiser ; Ingo Gregor ; Wolfgang Bö ; nigk ; Gü ; nther Schmalzing ; Jö ; rg Enderlein
- 刊名：Analytical Chemistry
- 出版年：2007
- 出版时间：June 1, 2007
- 年：2007
- 卷：79
- 期：11
- 页码：4040 - 4049
- 全文大小：611K
- 年卷期：v.79,no.11(June 1, 2007)
- ISSN：1520-6882
文摘
Fluorescence antibunching is a well-known technique fordetermining the number of independent emitters permolecule or molecular complex. It was rarely applied toautofluorescent proteins due to the necessity of collectinglarge numbers of fluorescence photons from a singlemolecule, which is usually impossible to achieve withrather photolabile autofluorescent proteins. Here, wemeasure fluorescence antibunching on molecules in solution, allowing us to accumulate data over a large numberof molecules. We use that method for determining anaverage stoichiometry of molecular complexes. The proposed method is absolute in the sense that it does notneed any calibration or referencing. We develop thenecessary theoretical background and check the methodon pure dye solutions and on molecular complexes withknown stoichiometry.