Electrochemical Protein Chip with Arrayed Immunosensors with Antibodies Immobilized in a Plasma-Polymerized Film
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- 作者：Kenichi Kojima ; Atsunori Hiratsuka ; Hiroaki Suzuki ; Kazuyoshi Yano ; Kazunori Ikebukuro ; and Isao Karube
- 刊名：Analytical Chemistry
- 出版年：2003
- 出版时间：March 1, 2003
- 年：2003
- 卷：75
- 期：5
- 页码：1116 - 1122
- 全文大小：253K
- 年卷期：v.75,no.5(March 1, 2003)
- ISSN：1520-6882
文摘
An electrochemical protein chip was microfabricated. Athin-film three-electrode system, including an array of 36platinum working electrodes, a set of thin-film Ag/AgClelectrodes, and platinum auxiliary electrodes, was integrated on a glass substrate. Capture antibodies wereimmobilized in a 4.5-nm-thick double layer of a hexamethyldisiloxane plasma-polymerized film. Because oftheir highly cross-linked network structure, the captureantibodies could be firmly immobilized. No nonspecificadsorption was observed during a series of proceduresto detect target proteins, and electrochemical cross talkbetween neighboring sites was negligible. The sandwichimmunoassay was conducted on a single chip using modelproteins, 
-1-fetoprotein and 
2-microglobulin. A distinctcurrent increase following the oxidation of hydrogenperoxide produced by the enzymatic reaction of glucoseoxidase was observed, which indicates that the captureproteins could actually bind the target proteins. Two kindsof protein were detected independently on multiple siteswith respective capture antibodies.
-1-fetoprotein and 2-microglobulin. A distinctcurrent increase following the oxidation of hydrogenperoxide produced by the enzymatic reaction of glucoseoxidase was observed, which indicates that the captureproteins could actually bind the target proteins. Two kindsof protein were detected independently on multiple siteswith respective capture antibodies.