Preparation and Characterization of Nanoparticles Containing Trypsin Based on Hydrophobically Modified Chitosan

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• 作者：Chen-Guang Liu ; Kashappa Goud H. Desai ; Xi-Guang Chen ; and Hyun-Jin Park
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：March 9, 2005
• 年：2005
• 卷：53
• 期：5
• 页码：1728 - 1733
• 全文大小：170K
• 年卷期：v.53,no.5(March 9, 2005)
• ISSN：1520-5118

文摘

Trypsin was immobilized on linolenic acid modified chitosan using glutaraldehyde (GA) as cross-linker, which was confirmed by Fourier transform infrared (FTIR) spectra. The chitosan nanoparticlescontaining trypsin (TR) can be prepared after the sonication of immobilized trypsin. The GAconcentration affected both the enzyme activity of the nanoparticle and particle size. Results indicatedthat the activity of trypsin immobilized onto linolenic acid modified chitosan nanoparticles increasedwith increasing concentration of GA up to 0.07% (v/v) and then decreased with increasing amount ofGA. On the other hand, particle size increased (from 523 to 1372 nm) with the increasing concentrationof GA (from 0.03 to 0.1% v/v). The enzyme catalytic characteristics of nanoparticle solution werealso studied. The results showed that the kinetic constant value (K_m) of TR immobilized on nanoparticle(71.9 mg/mL) was higher than that of pure TR (50.2 mg/mL). However, the thermal stability andoptimum temperature of TR immobilized on nanoparticles improved, which make it more attractive inthe application aspect.Keywords: Chitosan; nanoparticles; trypsin; hydrophobically modified chitosan