Linolenic Acid-Modified Chitosan for Formation of Self-Assembled Nanoparticles

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• 作者：Chen-Guang Liu ; Kashappa Goud H. Desai ; Xi-Guang Chen ; and Hyun-Jin Park
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：January 26, 2005
• 年：2005
• 卷：53
• 期：2
• 页码：437 - 441
• 全文大小：174K
• 年卷期：v.53,no.2(January 26, 2005)
• ISSN：1520-5118

文摘

Chitosan was modified by coupling with linolenic acid through the 1-ethyl-3-(3-dimethylaminopropyyl)carbodiimide-mediated reaction. The degree of substitution was measured by ¹H NMR, and it was1.8%, i.e., 1.8 linolenic acids group per 100 anhydroglucose units. The critical aggregationconcentration (CAC) of the self-aggregate of hydrophobically modified chitosan was determined bymeasuring the fluorescence intensity of the pyrene as a fluorescent probe. The CAC value inphosphate-buffered saline (PBS) solution (pH 7.4) was 5 × 10^-2 mg/mL. The average particle sizeof self-aggregates of hydrophobically modified chitosan in PBS solution (pH 7.4) was 210.8 nm witha unimodal size distribution ranging from 100 to 500 nm. A transmission electron microscopy studyshowed that the formation of near spherical shape nanoparticles had enough structural integrity.The loading ability of hydrophobically modified chitosan (LA-chitosan) was investigated by using bovineserum albumin (BSA) as a model protein. Self-aggregated nanoparticles exhibited an increased loadingcapacity (19.85 ± 0.04 to 37.57 ± 0.25%) with an increasing concentration of BSA (0.1-0.5 mg/mL).Keywords: Chitosan; linolenic acid; nanoparticles; self-aggregates; BSA