Screening of Indonesian Medicinal Plant Extracts for Antibabesial Activity and Isolation of New Quassinoids from Brucea javanica

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• 作者：Subeki ; Hideyuki Matsuura ; Kosaku Takahashi ; Kensuke Nabeta ; Masahiro Yamasaki ; Yoshimitsu Maede ; Ken Katakura
• 刊名：Journal of Natural Products
• 出版年：2007
• 出版时间：October 2007
• 年：2007
• 卷：70
• 期：10
• 页码：1654 - 1657
• 全文大小：77K
• 年卷期：v.70,no.10(October 2007)
• ISSN：1520-6025

文摘

Boiled extracts derived from 28 Indonesian medicinal plants were screened for their antibabesial activity against Babesia gibsoni in vitro. Of these extracts, the fruit of Brucea javanica was the most active in inhibiting parasite growth at a concentration of 10 µg/mL. Bioassay-guided fractionation of the fruit extract of Br. javanica led to the isolation of two new quassinoids, bruceantinol B and bruceine J, and the structures of these compounds were elucidated on the basis of their spectroscopic data and by chemical transformation to known compounds. In addition, the known quassinoids bruceines A–D, bruceantinol, and yadanziolide A were isolated. Antibabesial activities were also examined in vitro, and bruceine A and bruceantinol were shown to be more potent than diminazene aceturate, a drug (IC₅₀ = 103 ng/mL) used clinically against B. gibsoni, with IC₅₀ values of 4 and 12 ng/mL, respectively.