Noncovalent Dimer Formation in Liquid Chromatography鈥揗ass Spectrometry Analysis
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- 作者：Katalin F. Medzihradszky
- 刊名：Analytical Chemistry
- 出版年：2014
- 出版时间：September 16, 2014
- 年：2014
- 卷：86
- 期：18
- 页码：8906-8909
- 全文大小：219K
- ISSN：1520-6882
文摘
Evidence will be presented that in the article 鈥淣ovel LC鈥揗S2 Product Dependent Parallel Data Acquisition Function and Data Analysis Workflow for Sequencing and Identification of Intact Glycopeptides鈥?written by Wu, S.-W.; Pu, T.-H.; Viner, R.; Khoo, K.-H. in Anal. Chem. 2014, 86, 5478鈥?/x> 5486, noncovalent homo- and heterodimers were mis-identified as glycopeptides bearing well-defined N-linked structures, where the unexplained mass was attributed to excessive O-glycosylation. Noncovalent dimer formation of abundant components has not previously been considered as a complication in high-throughput proteomic analyses.