Measuring Markers of Liver Function Using a Micropatterned Paper Device Designed for Blood from a Fingerstick
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- 作者：Sarah J. Vella ; Patrick Beattie ; Rebecca Cademartiri ; Anna Laromaine ; Andres W. Martinez ; Scott T. Phillips ; Katherine A. Mirica ; George M. Whitesides
- 刊名：Analytical Chemistry
- 出版年：2012
- 出版时间：March 20, 2012
- 年：2012
- 卷：84
- 期：6
- 页码：2883-2891
- 全文大小：440K
- 年卷期：v.84,no.6(March 20, 2012)
- ISSN：1520-6882
文摘
This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and 鈥渟piked鈥?standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.