Selective Deposition and Self-Assembly of Triblock Copolymers into Matrix Arrays for Membrane Protein Production

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• 作者：Mirjam Andreasson-Ochsner ; Zhikang Fu ; Sylvia May ; Low Ying Xiu ; Madhavan Nallani ; Eva-Kathrin Sinner
• 刊名：Langmuir
• 出版年：2012
• 出版时间：January 31, 2012
• 年：2012
• 卷：28
• 期：4
• 页码：2044-2048
• 全文大小：310K
• 年卷期：v.28,no.4(January 31, 2012)
• ISSN：1520-5827

文摘

To improve the stability of cell membrane mimics, there has been growing interest in the use of block copolymers. Here, we present an easy approach to create an array of planar polymeric matrices capable of hosting membrane proteins. The array of polymeric matrices was formed by the selective deposition of triblock copolymers onto an array of hydrophilic islands situated within a hydrophobic background. The thickness of these matrices corresponds to the length of a single polymer chain. These polymeric matrices were used to host cell-free expressed membrane proteins, and offers a prototype from which a membrane protein array can be created for diagnostics or drug discovery purposes.