Alkyne-Tag Raman Imaging for Visualization of Mobile Small Molecules in Live Cells

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• 作者：Hiroyuki Yamakoshi ; Kosuke Dodo ; Almar Palonpon ; Jun Ando ; Katsumasa Fujita ; Satoshi Kawata ; Mikiko Sodeoka
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：December 26, 2012
• 年：2012
• 卷：134
• 期：51
• 页码：20681-20689
• 全文大小：580K
• 年卷期：v.134,no.51(December 26, 2012)
• ISSN：1520-5126

文摘

Alkyne has a unique Raman band that does not overlap with Raman scattering from any endogenous molecule in live cells. Here, we show that alkyne-tag Raman imaging (ATRI) is a promising approach for visualizing nonimmobilized small molecules in live cells. An examination of structure鈥揜aman shift/intensity relationships revealed that alkynes conjugated to an aromatic ring and/or to a second alkyne (conjugated diynes) have strong Raman signals in the cellular silent region and can be excellent tags. Using these design guidelines, we synthesized and imaged a series of alkyne-tagged coenzyme Q (CoQ) analogues in live cells. Cellular concentrations of diyne-tagged CoQ analogues could be semiquantitatively estimated. Finally, simultaneous imaging of two small molecules, 5-ethynyl-2鈥?deoxyuridine (EdU) and a CoQ analogue, with distinct Raman tags was demonstrated.