Determination of Benzo[a]pyrene-7,10-quinone in Airborne Particulates by Using a Chemiluminescence Reaction of Hydrogen Peroxide and Hydrosulfite

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• 作者：Ruibo Li ; Takayuki Kameda ; Akira Toriba ; Kazuichi Hayakawa ; Jin-Ming Lin
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：April 3, 2012
• 年：2012
• 卷：84
• 期：7
• 页码：3215-3221
• 全文大小：392K
• 年卷期：v.84,no.7(April 3, 2012)
• ISSN：1520-6882

文摘

An ultraweak chemiluminescence (CL) was observed when sodium hydrosulfite (NaHSO₃) reacts with hydrogen peroxide (H₂O₂) and was enhanced 70 times by adding 10 pmol benzo[a]pyrene-7,10-quinone (7,10-BaPQ). The CL reaction is fast, and it reached maximum intensity in 0.1 s, and then decayed to baseline in 3 s. Mechanism of NaHSO₃-7,10-BaPQ-H₂O₂ system were investigated by CL spectrum, radical scavengers and electron spin resonance (ESR). Hydroxyl radical (^{鈥?/sup>OH), super oxide anion radical (鈥?/sup>O₂鈥?/sup>), and sulfite radical (鈥?/sup>SO₃鈥?/sup>) were generated in the NaHSO₃-7, 10-BaPQ-H₂O₂ system. Reduction of 7,10-BaPQ by 鈥?/sup>O₂鈥?/sup> radical gave excited semiquinone, which showed strong CL emission when decayed to its ground state. Maximum CL emission wavelength was located at 440 nm, which may belong to the excited semiquinone. This CL system was developed as post column detection of high performance liquid chromatography for the determination of 7,10-BaPQ. Linearity ranged from 50 fmol to 20 pmol (R2 = 0.9995) with limit of detection of 30 fmol (S/N = 3). The proposed method was used to determine 7,10-BaPQ in airborne particulates. Average atmospheric concentrations of 7,10-BaPQ in Kanazawa in December 2010 and Wajima in October 2007 were 2.0 and 1.6 pg/m3, respectively.}