Concentration of Hydrophobic Organic Compounds by Polymer-Mediated Extraction
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- 作者：Tohru Saitoh ; Yuki Yoshida ; Toshiyuki Matsudo ; Shinsuke Fujiwara ; Akira Dobashi ; Kazuo Iwaki ; Yasuzo Suzuki ; and Chiyo Matsubara
- 刊名：Analytical Chemistry
- 出版年：1999
- 出版时间：October 15, 1999
- 年：1999
- 卷：71
- 期：20
- 页码：4506 - 4512
- 全文大小：73K
- 年卷期：v.71,no.20(October 15, 1999)
- ISSN：1520-6882
文摘
A water-soluble polymer, poly(N-isopropylacrylamide),becomes water-insoluble above a lower critical solutiontemperature (~32 
C) and forms gumlike precipitates(polymer phase) having a very small volume fraction. Thefeatures in the polymer precipitation were dependent onthe molecular weight of the polymer and/or the concentration of salts or buffer components. Accompanying theformation of the polymer phase, hydrophobic organiccompounds including polycyclic aromatic hydrocarbons,alkylphenols, chlorobenzenes, chlorophenols, phthalicesters, pesticides, and steroid hormones in water wereefficiently incorporated into the polymer phase. On theother hand, hydrophilic components such as inorganicions or polysaccharides remained in the bulk aqueoussolution. The extractabilities of the compounds increasedwith increasing hydrophobic properties. The distributioncoefficients, based on the volume fractions of their aqueous and polymer phases, between the bulk aqueous andpolymer phases were correlated with the water-octanoldistribution coefficients. The linear relationship betweenthe two equilibrium coefficients indicated that the hydrophobic interaction is the predominating force for incorporating solutes. The introduction of the concentratedpolymer solution into the hygroorganic mobile phase ofthe reversed-phase HPLC rarely interfered with the chromatographic operation. The application of the polymer-mediated extraction to HPLC significantly enhanced thepeak intensities of the hydrophobic analyte varieties byreducing the interferences of the hydrophobic matrixcomponents.
C) and forms gumlike precipitates(polymer phase) having a very small volume fraction. Thefeatures in the polymer precipitation were dependent onthe molecular weight of the polymer and/or the concentration of salts or buffer components. Accompanying theformation of the polymer phase, hydrophobic organiccompounds including polycyclic aromatic hydrocarbons,alkylphenols, chlorobenzenes, chlorophenols, phthalicesters, pesticides, and steroid hormones in water wereefficiently incorporated into the polymer phase. On theother hand, hydrophilic components such as inorganicions or polysaccharides remained in the bulk aqueoussolution. The extractabilities of the compounds increasedwith increasing hydrophobic properties. The distributioncoefficients, based on the volume fractions of their aqueous and polymer phases, between the bulk aqueous andpolymer phases were correlated with the water-octanoldistribution coefficients. The linear relationship betweenthe two equilibrium coefficients indicated that the hydrophobic interaction is the predominating force for incorporating solutes. The introduction of the concentratedpolymer solution into the hygroorganic mobile phase ofthe reversed-phase HPLC rarely interfered with the chromatographic operation. The application of the polymer-mediated extraction to HPLC significantly enhanced thepeak intensities of the hydrophobic analyte varieties byreducing the interferences of the hydrophobic matrixcomponents.