Surface-Independent Antibacterial Coating Using Silver Nanoparticle-Generating Engineered Mussel Glue
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- 作者：Yun Kee Jo ; Jeong Hyun Seo ; Bong-Hyuk Choi ; Bum Jin Kim ; Hwa Hui Shin ; Byeong Hee Hwang ; Hyung Joon Cha
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2014
- 出版时间：November 26, 2014
- 年：2014
- 卷：6
- 期：22
- 页码：20242-20253
- 全文大小：643K
- ISSN：1944-8252
文摘
During implant surgeries, antibacterial agents are needed to prevent bacterial infections, which can cause the formation of biofilms between implanted materials and tissue. Mussel adhesive proteins (MAPs) derived from marine mussels are bioadhesives that show strong adhesion and coating ability on various surfaces even in wet environment. Here, we proposed a novel surface-independent antibacterial coating strategy based on the fusion of MAP to a silver-binding peptide, which can synthesize silver nanoparticles having broad antibacterial activity. This sticky recombinant fusion protein enabled the efficient coating on target surface and the easy generation of silver nanoparticles on the coated-surface under mild condition. The biosynthesized silver nanoparticles showed excellent antibacterial efficacy against both Gram-positive and Gram-negative bacteria and also revealed good cytocompatibility with mammalian cells. In this coating strategy, MAP-silver binding peptide fusion proteins provide hybrid environment incorporating inorganic silver nanoparticle and simultaneously mediate the interaction of silver nanoparticle with surroundings. Moreover, the silver nanoparticles were fully synthesized on various surfaces including metal, plastic, and glass by a simple, surface-independent coating manner, and they were also successfully synthesized on a nanofiber surface fabricated by electrospinning of the fusion protein. Thus, this facile surface-independent silver nanoparticle-generating antibacterial coating has great potential to be used for the prevention of bacterial infection in diverse biomedical fields.