Controlling the Material Properties and Biological Activity of Lipase within Sol-Gel Derived Bioglasses via Organosilane and Polymer Doping
详细信息 查看全文
- 作者：Tracey Keeling-Tucker ; Michael Rakic ; Cassandra Spong ; and John D. Brennan
- 刊名：Chemistry of Materials
- 出版年：2000
- 出版时间：December 2000
- 年：2000
- 卷：12
- 期：12
- 页码：3695 - 3704
- 全文大小：135K
- 年卷期：v.12,no.12(December 2000)
- ISSN：1520-5002
文摘
The development of optical biosensors based on sol-gel entrapped proteins requires adetailed understanding of the evolution of the physicochemical properties of the material,their affects on protein function, and how these factors can be tailored by processingconditions. In this study, the polymer additives poly(vinyl alcohol) (PVA) and poly(ethyleneglycol) (PEG) were dispersed into sol-gel processed materials derived from tetraethylorthosilicate (TEOS) alone or copolymerized with methyltriethoxysilane (MTES) or dimethyldimethoxysilane (DMDMS), and their effects on the chemical and physical properties of thematerials were monitored. In general, the physical properties, including transmittance andresistance to cracking, improved with increasing PEG concentration, but deteriorated withPVA content. The spectroscopic data obtained from entrapped 7-azaindole and 6-propionyl-2-(dimethylamino)naphthalene suggested that the inclusion of polymers and organic moietiesinto the matrix affected both the homogeneity of the materials and the polarity of the internalenvironment, with PEG reducing and PVA increasing the internal polarity. In light of theseresults, preliminary studies were performed on the effects of organic and polymer contenton the initial and long-term activity of entrapped lipase. Concomitant with the materialdata, PVA tended to have a detrimental affect on lipase activity, while PEG provided aconcentration-dependent enhancement of the enzyme activity. This study demonstrates forthe first time that durable, optically transparent materials with significant lipase activitycan be prepared and that optimal materials are produced with TEOS as a precursor and afew weight percent of low molecular weight PEG as an additive, with no need for organosilaneprecursors.