Purification and Identification of an ACE Inhibitory Peptide from Walnut Protein

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• 作者：Meng Liu ; Ming Du ; Yingchun Zhang ; Weili Xu ; Cong Wang ; Kejian Wang ; Lanwei Zhang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：May 1, 2013
• 年：2013
• 卷：61
• 期：17
• 页码：4097-4100
• 全文大小：195K
• 年卷期：v.61,no.17(May 1, 2013)
• ISSN：1520-5118

文摘

In the present study, a novel angiotensin I-converting enzyme (ACE)-inhibitory peptide, P-2a2, was purified to homogeneity from walnut protein hydrolysate by ultrafiltration, consecutive column chromatography, and high-performance liquid chromatography. The purified peptide was characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrophotometry and a liquid-phase peptide sequencer. The molecular mass of P-2a2 was tested to be 1033.42 D. Its amino acid sequence was determined to be Trp-Pro-Glu-Arg-Pro-Pro-Gln-Ile-Pro. The potent ACE-inhibitory peptide is an enneapeptide and shows a high ACE-inhibitory activity, with an IC₅₀ value of 25.67 渭g/mL.

Keywords:

walnut; peptide; ACE-inhibitory activity; purification; characterization