Vascular Smooth Muscle Cells on Polyelectrolyte Multilayers: Hydrophobicity-Directed Adhesion and Growth

详细信息    查看全文

• 作者：David S. Salloum ; Scott G. Olenych ; Thomas C. S. Keller ; and Joseph B. Schlenoff
• 刊名：Biomacromolecules
• 出版年：2005
• 出版时间：January 2005
• 年：2005
• 卷：6
• 期：1
• 页码：161 - 167
• 全文大小：344K
• 年卷期：v.6,no.1(January 2005)
• ISSN：1526-4602

文摘

Polyelectrolyte multilayer films were employed to support attachment of cultured rat aortic smooth muscleA7r5 cells. Like smooth muscle cells in vivo, cultured A7r5 cells are capable of converting between anonmotile "contractile" phenotype and a motile "synthetic" phenotype. Polyelectrolyte films were designedto examine the effect of surface charge and hydrophobicity on cell adhesion, morphology, and motility. Thehydrophobic nature and surface charge of different polyelectrolyte films significantly affected A7r5 cellattachment and spreading. In general, hydrophobic polyelectrolyte film surfaces, regardless of formal charge,were found to be more cytophilic than hydrophilic surfaces. On the most hydrophobic surfaces, the A7r5cells adhered, spread, and exhibited little indication of motility, whereas on the most hydrophilic surfaces,the cells adhered poorly if at all and when present on the surface displayed characteristics of being highlymotile. The two surfaces that minimized cell adhesion consisted of two varieties of a diblock copolymercontaining hydrophilic poly(ethylene oxide) and a copolymer bearing a zwitterionic group AEDAPS, (3-[2-(acrylamido)-ethyldimethyl ammonio] propane sulfonate). Increasing the proportion of AEDAPS in thecopolymer decreased the adhesion of cells to the surface. Cells presented with micropatterns of cytophilicand cytophobic surfaces generated by polymer-on-polymer stamping displayed a surface-dependentcytoskeletal organization and a dramatic preference for adhesion to, and spreading on, the cytophilic surface,demonstrating the utility of polyelectrolyte films in manipulating smooth muscle cell adhesion and behavior.