Effective Tolerance to Serum Proteins of Head−Tail Type Polycation Vectors by PEGylation at the Periphery of the Head Block

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• 作者：Atsushi Harada ; Yuka Kimura ; Chie Kojima ; Kenji Kono
• 刊名：Biomacromolecules
• 出版年：2010
• 出版时间：April 12, 2010
• 年：2010
• 卷：11
• 期：4
• 页码：1036-1042
• 全文大小：266K
• 年卷期：v.11,no.4(April 12, 2010)
• ISSN：1526-4602

文摘

To improve the stability of a head−tail type polycation (PAMAM dendron-PLL), which is composed of a polyamidoamine (PAMAM) dendron head and poly(l-lysine) (PLL) tail blocks, as a gene vector against serum proteins, the PEGylation of the PAMAM dendron head was examined using physicochemical and transfection experiments. The PEGylation of the PAMAM dendron-PLL polyplexes neutralized the zeta-potential, indicating that the PEG brushes surround the surface of the polyplexes. The PEGylated PAMAM dendron-PLL polyplexes had higher tolerance against incubation with serum proteins when compared with non-PEGylated PAMAM dendron-PLL polyplexes. In addition, the transfection efficiency of the PEGylated PAMAM dendron-PLL polyplexes increased with increasing incubation periods, leading to an increase in the amount of pDNA uptaked by HeLa cells. Furthermore, effective stabilization of the PAMAM dendron-PLL polyplexes through PEGylation of the PAMAM dendron block was confirmed by the transfection experiments after preincubation with serum proteins and the PEGylated PAMAM dendron-PLL polyplexes showed a comparable level of transfection efficiency with polyethyleneimine polyplexes.